I am trying to use a protocol off a paper to isolate DNA. It's standart phenol:chloroform:iaa extraction followed by ethanol precipitation. So far I haven't been successful. I think the problem is in lysis step. Because the paper claims that sometimes samples completely dissolve in lysis buffer. But mine don't. Furthermore, when I proceed to phenol:chloroform:iaa I see no interphase indicating no protein. The lysis buffer in the paper is as follows:
Edited by burakkc, 25 November 2013 - 04:53 AM.