I recemy;y extracted cytoplasmic protein from a 10cm dish using the NE-PER ® Nuclear and Cytoplasmic Extraction kit (78833 ).
However, my protein is too concentrated to run for western. I need to run 5ug-10ug for western which is at the moment equivalent to 1ul of my concentrated sample. This is too concentrated for running western. How can I dilute the protein? I was thinking of adding some additional cytoplasmic extraction buffer that came with the Kit?