Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

red dots in cell culture line?


  • Please log in to reply
3 replies to this topic

#1 luckyjade

luckyjade

    member

  • Active Members
  • Pip
  • 21 posts
1
Neutral

Posted 24 November 2013 - 09:29 PM

Hello,

 

Today I checked on my plates that I have been taking care of for the last two months or so. There were clearly visible red dots that appeared in the plate (without looking with a microscope.) After looking under a microscope, they looked much bigger compared to the "correct" cells. Does anyone know what these are? And why they are red...?



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,512 posts
371
Excellent

Posted 25 November 2013 - 12:04 AM

The red colour is probably a pigment of some sort, exactly which bacterium or fungus this is likely to be is hard to say, but it could be Serratia marcescens.  However, this will depend on the medium you are using, your location, temperature, etc.



#3 CellApplicationsInc

CellApplicationsInc

    Sr. Product Scientist

  • Active Members
  • Pip
  • 21 posts
2
Neutral

Posted 25 November 2013 - 08:56 AM

are your "correct" cells still there? It is possible that the red is the precipitate of the Phenol Red containing medium, which could happen if the media partiatially dehydrates. Do you still have water in the tray of your incubator?

Is it possible that your media is just slowly drying out? Also if you can post a picture of your culture, that would help us to figure out what goes on.



#4 luckyjade

luckyjade

    member

  • Active Members
  • Pip
  • 21 posts
1
Neutral

Posted 28 November 2013 - 12:00 AM

thank you so much for your responses! unfotunately i got rid of the plate already. however there was still water in the tray of the incubator and the correct cells were still there






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.