Dear Colleagues, I have a simple question: what is the optimal time for lentivirus transduction efficiency measuring (eg. by GFP)?
Transducted cells need not so much time to express the GFP protein, but why in most cases researchers measure the efficiency after 12-48-72 h., but not in 6 h., for example? Why we need to wait so long?? Mammalian cells, which was not transducted, divided approximately 4-8 times in this long time, for this reason the efficiency of transducted cells in % will be less after 72 h. then after 6 h., am I wright?
SO, what is the logic in choosing such time points?
Edited by ticovka, 23 November 2013 - 11:08 AM.