We have recently bought a Qubit from LifeTechnologies. We use dsDNA HS kit in with 195 ul working solution 5 ul DNA. Standards are prepared according to the instructions. Many of the samples we\ve measured were however lower than expected and we are worried that something is wrong.
1. For a few samples we had qPCR estimates from a few years back and included these from comparison. They differ considerably, though.
Sample 1 qPCR 9.1 ng/ul Qubit 2.1 ng/ug
Sample 2 qPCR 10.9 ng/ul, Qubit 3.2 ng/ul
Has someone else experienced these problems?
Also, we unaccidentally stored the dsDNA HS reagents in the freezer for the first 24 hours, Could that be the cause of our weird results?