problem with COV318 ovarian cancer cell line
Posted 22 November 2013 - 11:48 AM
Posted 23 November 2013 - 11:52 PM
Sounds like there is probably some sort of Hayflick limit approaching. Don;t know about the cell line in particular though.
COuld be some sort of hormone effect - are you using phenol red in your culture medium? If so, it is an estrogen analogue, which means that ER+ cells will internalize the receptor and slow growth rates.
Posted 25 November 2013 - 02:56 AM
Posted 25 November 2013 - 01:05 PM
I think it would be worth using phenol red free medium, though the original paper (pdf here) just used DMEM with l-glutamine (3 mM) and l-asparagine (1 mM). I still think they are likely to be approaching the Hayflick limit, as they are essentially a primary line still, in which case there won't be anything you can do to prevent it happening.
Sigma sell them, so you could approach their technical support to see what they have to say.
Posted 22 January 2014 - 07:20 AM
Hi I am working with COV318 cells too. I just bought them from Sigma and they were Passage 44!bongiwoman what passage are your cells now if I may ask? Are they indeed senescent? I am kind of worried about the Hayflick limit myself since a lot of my cells are senescent since I revived them..