timecourse experiment for Western and qPCR
Posted 22 November 2013 - 11:32 AM
Posted 23 November 2013 - 05:40 AM
Presumably you are doing these tests to explain some physiological phenomenon you observed at the later time point, so you should handle the cells precisely as you did for the phenotypic experiment. If instead you are at the phase of optimizing knockdown efficiency at the later time point so that you might see a phenotypic difference later on, then just try every possibility and see what works best. I personally think that plating very few cells (10% confluency) at the beginning is a good approach, but it all depends on what you are trying to test.
Posted 25 November 2013 - 02:42 AM