I have never doen Co-IP and would have to perform one soon. I would appreciate all advice in this regard.
I want to check if my drug of interest affects the GSK3B/Axin/b-catenin complex. But I’m not sure where to start. Do I pull down with GSK3b and check if Axin and b-catenin are holding on? Also, can all mono clonal antibdodies be used for Co-IP? I have GSK3B and Axin from Cell Signalling and the manual says they can be used for IP?