Am designing an experiment to check the effect of a substance on the viral infection using a cell line grown in 96-well plate. The main aim is to see if the infection rate increases or decreases at a single concentration of the substance. Now there are two ways to design this experiment.
1) you seed cells in 96-well plate and dilute down the virus starting at 1:50 dilution all the way to 1:32000 along with a starting concentration of the substance. This essentially means the substance will get diluted but will be equally diluted along with the virus.
2) you seed cells in 96-well plate and dilute down the virus starting at 1:50 dilution all the way to 1:32000. And then add an equal concentration i.g: 10ug/ul of the substance through out the dilutions. This would essentially mean that 100 virus or 10 virus (as you go down the dilution) will be subjected to 10ug/ul.
Which one is correct, I would think both needs to be done to eliminate the drawbacks but there is very little of the substance.
The analysis of viral infection will be carried out using flow cytometry