I am in need of clarification of something that is concerning me. I am wanting to extract a transmembrane protein localised in the cytoplasmic membrane of E. coli for western blotting and hence I bought B Per from Pierce Scientific. It states that it is an easy to use, one reagent method for gentle lysis of bacterial cells to extract both soluble and insoluble proteins and soluble proteins from inclusion bodies. However, it does not specifically state that it does total protein extraction and hence the transmembrane proteins.
Can anyone put my mind at ease that this solution will extract membrane bound proteins, or do I need to write this off as an extravegant and needless purchase which I will need to get another kit that is more suitable for the job.
Any help on this matter would be very appreciated.