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in-competent cells


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#1 paddy

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Posted 09 May 2004 - 10:03 AM

Hi, I'm trying to transform ultra-competent cells (XL10) but to no avail.... there are only ever a couple of colonies on the plate and even the puc18 control will not work. I've followed the protocol, so what could be up?

#2 unknownphd

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Posted 10 May 2004 - 02:30 PM

Sounds like you figured it out.. incompetent. Were they always stored at -80? I would try getting an aliquot from somewhere else and do a parallel reaction with your cells to confirm this.




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