I don't know if it is my fuzzy memory or not but I think that I've read that a recombinant protein that terminates in a proline residue is at an increased risk of degradation and subsequent apparent low yield. Am I making this up or has someone else heard of this too. I've been searching for a reference to confirm and haven't been able to come up with one. I'm about to start a new recombinant project and the protein ends with a double proline residue. I need to decide if I want to cap it in some fashion or if its not really necessary.
Thanks in advance for the help!