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Extremely detailed SYBR green standard curve qPCR protocol

qpcr qRTPCR SYBR standard curve RNA quality

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#1 doxorubicin

doxorubicin

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Posted 20 November 2013 - 03:28 AM

In response to some of the questions that I've seen on this forum, I've prepared and posted an extremely detailed qPCR SYBR green standard curve protocol here (on our alternative careers site):

 

https://sites.google...n-qpcr-protocol

 

It includes everything from assessing RNA quality, testing the specificity of your primers, creating and testing the standard curve, and how to move from one step to the next to generate meaningful results.

 

Similarly, I've prepared a protocol for designing qPCR primers:

 

https://sites.google...wn-qpcr-primers

 

I hope this information is helpful.

 

 







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