Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Extremely detailed SYBR green standard curve qPCR protocol

qpcr qRTPCR SYBR standard curve RNA quality

  • Please log in to reply
No replies to this topic

#1 doxorubicin



  • Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 193 posts

Posted 20 November 2013 - 03:28 AM

In response to some of the questions that I've seen on this forum, I've prepared and posted an extremely detailed qPCR SYBR green standard curve protocol here (on our alternative careers site):




It includes everything from assessing RNA quality, testing the specificity of your primers, creating and testing the standard curve, and how to move from one step to the next to generate meaningful results.


Similarly, I've prepared a protocol for designing qPCR primers:




I hope this information is helpful.



Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.