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DNA Liquid into DNA pellet

pellet dna dna extraction pellet

Best Answer hobglobin, 20 November 2013 - 09:14 AM

You can use different salts which depends a bit on the later application.

NaOAc is often a quite good choice but inhibits e.g. Klenow fragment.Other salts have different effects. 

Consider the recommended amounts and concentration of the chemicals and best is to follow an established protocol (there are many in the web e.g. this or this.)

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#1 detriar

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Posted 20 November 2013 - 01:11 AM

Dear all,

 

I extracted DNA from frozen tissue using Qiagen kit, and I got the final DNA result as liquid, as it was eluted with the elution buffer. But then my PI asked me to turn it into pellet. Does anyone here know how to do it, considering I've already reached the last step using the spin column and all?

 

Thank you.

Cheers,

detriar



#2 bob1

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Posted 20 November 2013 - 01:16 AM

Precipitate the DNA - add some salt and ethanol or isopropanol, incubate, spin down, dry.



#3 detriar

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Posted 20 November 2013 - 01:20 AM

Dear bob1,

is the salt considered as NaOAc? I've read it somewhere, but I can't be sure about that before I asked.

 

Thank you.



#4 hobglobin

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Posted 20 November 2013 - 09:14 AM   Best Answer

You can use different salts which depends a bit on the later application.

NaOAc is often a quite good choice but inhibits e.g. Klenow fragment.Other salts have different effects. 

Consider the recommended amounts and concentration of the chemicals and best is to follow an established protocol (there are many in the web e.g. this or this.)


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#5 detriar

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Posted 20 November 2013 - 11:40 PM

Thank you all, it's very helpful.smile.png







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