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miRNA overexpression plasmid

miRNA Normalisation Luc cotransfection

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#1 Sabna Cmn

Sabna Cmn

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Posted 20 November 2013 - 12:16 AM

Hi all,

 

Just a technical question. How could I use the RLUs derived from a Luc reporter plasmid to normalise the transfection efficiency

of an miRNA overexpressing plasmid? Its a co-transfection approach as I don't have both the elements (reporter and the miRNA gene)

on the same plasmid.

 

Thanks!

Sabna







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