I have a little difficulties with rna concentration. I extracted rna from frozen tumor tissue, grind it with liquid N2, using RNeasy Qiagen kit. Usually, I got concentration around 200-300 ng/ul, and good ratio 260/280 and 260/230 (around 2.00), but when I extracted from 'bloody' frozen tumor tissue (blood surrounding the frozen tissue), I got poor concentration (around 9-17 ng/ul). Can someone please help me with this problem? Is it my poor technique or the blood caused this?
Any help will do.
Edited by detriar, 19 November 2013 - 11:00 PM.