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RNA from 'bloody' frozen tissue

rnafrozen tissue tumor tissue poor concentration bloody tissue

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#1 detriar

detriar

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Posted 19 November 2013 - 10:59 PM

Hello all,

 

I have a little difficulties with rna concentration. I extracted rna from frozen tumor tissue, grind it with liquid N2, using RNeasy Qiagen kit. Usually, I got concentration around 200-300 ng/ul, and good ratio 260/280 and 260/230 (around 2.00), but when I extracted from 'bloody' frozen tumor tissue (blood surrounding the frozen tissue), I got poor concentration (around 9-17 ng/ul). Can someone please help me with this problem? Is it my poor technique or the blood caused this?

 

Any help will do.

 

Thank you.

Cheers,

detriar


Edited by detriar, 19 November 2013 - 11:00 PM.





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