I just joined this forum, because I need some advice on microscopy of stomata. I am designing a set of experiments for a student's course . One of these experiments contains measurement of stomata width connected with different light regimes. I wanted to use the "nail varnish technique". My preferred plant for this experiment is Cyclamen.
1) It takes some minutes until the varnish is hardened. Is it possible that in the "open" time the opening or closing of the stoma in respect to the light factors continues?
2) I am wondering if nail varnish (or it's solvent) has a physiological effect on the stoma, i.e. can nail varnish initiate the opening or closing of the stoma? My varnish does not visually destroy the cells, there are no brwown spots or similar.
Thanks for answering,