I am currently working with human keratinocytes. I seed them into Flasks and let them grow until I can use them for my experiments.
Some of the cultures are doing very well so far, but others at least seem a bit difficult.
They do not all have these "cobblestone" aspects. In some flasks all of the keratinocytes are just small and round, although attached (so I guess they must still be alive) and covering almost the entire flask.
As I haven't found any information about this "behaviour" of the keratinocytes, I wanted to ask you guys if per chance one of you can tell me what the hell is going on with these keratinocytes.
Perhaps someone has or knows about some kind of atlas where u can browse through all possible shapes of these cells?