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DEPC Treatment of Water

DEPC

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#1 melsmith

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Posted 18 November 2013 - 08:00 AM

I was wondering if anyone has tips on making DEPC treated water. I'm not sure how many precautions I should take when using it, I do however pipette the solution into my water in a fume hood. I usually prepare several liters at a time in separate containers, heat to 37C for 2 hours, then autoclave on a liquid cycle for 30 minutes, the whole cycle takes about an hour. Should this been good enough to inactivate the DEPC? thanks!



#2 phage434

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Posted 18 November 2013 - 08:57 AM

I usually add DEPC, shake, and leave overnight, then autoclave in the morning. Your approach almost certainly also works.







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