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ng calulation please


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2 replies to this topic

#1 alee

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Posted 16 November 2013 - 07:42 AM

Hi all,

I have checked my  DNA concentration on nanodrop which is 4 ng/ul. Total volume of my sample is 40ul. So, does it mean that I have 160ng of sample? I need 10ng of DNA from this sample for my further experiment.

 

160 x ? = 10X 15ul (final vol)

 

Is this calculation correct????

 

please let me know.....

 

Thanks Ale

 

 

 



#2 hobglobin

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Posted 16 November 2013 - 08:59 AM

160 ng is correct except that you might have other stuff such as RNA in it which also adds to "sample"  (if it's e.g. genomic DNA you isolated without removing RNA).

Your calculation I didn't get...

I'd calculate 4 ng = 1 uL, 10 ng = 2.5 uL.


Edited by hobglobin, 16 November 2013 - 09:22 AM.

One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 phage434

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Posted 16 November 2013 - 08:25 PM

Also, the nanodrop is not very accurate at the 4 ng/ul level. I'd expect at least a factor two possible error.






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