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His-tag and GST-tag


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#1 weilun

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Posted 15 November 2013 - 03:16 AM

Does anyone know why we cannot use glutathione bead to pull down His-tagged protein while glutatione can be used for GST-tagged?

 

thanks !



#2 Missle

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Posted 15 November 2013 - 05:01 AM

GST (Glutathione S-transferase) reacts specifically with glutathione and a Histidine tag does not.






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