What is the significance for using water soluble hydrocortisone vs hydrocortisone. I am not looking for an explanation of the molecular difference. Rather, I want to know why I would use one over the other in tissue culture or if they are interchangeable. I have regular hydrocortisone. Howevever, I recently acquired a cell line from another lab and their complete media requires water soluble hydrocortisone. Could I use one in replace of the other?