Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is there any one who is working with Thymoquinone ????


  • Please log in to reply
2 replies to this topic

#1 ReemooN

ReemooN

    member

  • Active Members
  • Pip
  • 8 posts
0
Neutral

Posted 13 November 2013 - 09:15 PM

Hi 

 

I need to work with Thymoquinone for in vitro experiment and I am having problems with dissolving it and preparing different concentrations of it to treat my cell lines. 

 

If any one has any information that can help I will be very grateful. 

 

Thanks 



#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,811 posts
136
Excellent

Posted 14 November 2013 - 04:45 AM

according to the merck index (ninth edition), thymoquinone is freely soluble in cold alcohol or ether; soluble in chloroform, benzene, hexane; very sparingly soluble in water.

 

in what are you trying to solubilize it?


talent does what it can
genius does what it must
i do what i get paid to do

#3 Osibisa

Osibisa

    member

  • Active Members
  • Pip
  • 10 posts
1
Neutral

Posted 15 January 2014 - 02:57 AM

I have used this before in the chem lab. It will only dissolve in HOT water, which is no use to you. Making an ethanol or DMSO stock and adding this to water is your best bet. What conc do you need? You should be able to go up to 1-2M in DMSO, but I think 100 uM in water will be your limit. Try this first before adding it to media. Also be sure to use mock treated cells with the same amount of DMSO as your control.

 

Oh and with these things keep in consideration the drug can impart volume to your stock. I.e. 1ml of a 1M stock may only be 900uL DMSO


Edited by Osibisa, 15 January 2014 - 02:59 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.