I have problems with separating three Proteins using SDS PAGE, and I Never had this Problems before! And i am at the end with my nerves! The MW of the Proteins Ranges between 20-24 kDA. I have always been able to nicely separate them using a 13% SdS gel. But not during the Last 3 Weeks! I exchanged all buffers, used New AA, but nothing happenend. Bands "stick" together and appear as one fat band! Do You have an idea? Thanks so much!
Edited by Sun86, 13 November 2013 - 02:41 PM.