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Sudden problems with Separating Proteins using SDS Gel

Protein Proteomics Gel electrophoresis

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4 replies to this topic

#1 Sun86

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Posted 13 November 2013 - 02:32 PM

Hi everyone,

I have problems with separating three Proteins using SDS PAGE, and I Never had this Problems before! And i am at the end with my nerves! The MW of the Proteins Ranges between 20-24 kDA. I have always been able to nicely separate them using a 13% SdS gel. But not during the Last 3 Weeks! I exchanged all buffers, used New AA, but nothing happenend. Bands "stick" together and appear as one fat band! Do You have an idea? Thanks so much!

Edited by Sun86, 13 November 2013 - 02:41 PM.


#2 Curtis

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Posted 13 November 2013 - 10:30 PM

make a 10% gel and retry



#3 mdfenko

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Posted 14 November 2013 - 04:38 AM

have you tried a fresh lot of sds?


talent does what it can
genius does what it must
i do what i get paid to do

#4 MacGyver

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Posted 13 April 2014 - 05:17 PM

curious to know what happened then....cause I am gonna separate low MW protein near future and i am very afraid of that...



#5 mdfenko

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Posted 14 April 2014 - 04:10 AM

curious to know what happened then....cause I am gonna separate low MW protein near future and i am very afraid of that...

you can separate low molecular weight proteins and peptides with tris-tricine gels (shagger and von jagow). for the formulation search the forum for "tricine".


talent does what it can
genius does what it must
i do what i get paid to do





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