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E coli. and Staph identification problem

e coli problem ident

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#1 Rjay

Rjay

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Posted 13 November 2013 - 07:13 AM

Hello,

 

I have been given e coli, staphylococcus aureus and a selection of agars (see below) and I have to come up with an order in which I would culture them to start determining that the bacteria are in fact e coli and staphylococcus aureus as if I had no idea.

 

The agars are,

  • Blood agar (incubate at 37oC overnight)
  • Baird Parker agar (incubate at 37oC overnight)
  • Slanetz and Bartley agar (incubate at 37oC overnight)
  • CLED agar (incubate at 37oC overnight)
  • Mueller Hinton agar (incubate at 37oC overnight)
  • Sabouraud agar (incubate at 25oC for 3 days)
  • TSC agar (incubate anaerobically at 37oC overnight)
  • Nutrient broth (incubate at 37oC overnight)

​I am able to use the oxoid to determine what each of these do however, I have no idea how I'd use them to determine e coli or staph if I didn't know what they were from the beginning,

 

Any help would be massively appreciated!!!

 

Kind Regards,

 

RJay



#2 bob1

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Posted 13 November 2013 - 11:50 AM

So what do the different media do?  Are some differential and some selective (or both?)






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