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#1 samita



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Posted 13 November 2013 - 04:04 AM

Does someone know how long is doxycyline is stable (as a inducer) in the cell culture. Or If I have to prolong the expression should I change the media after 24 hrs and used the fresh media with fresh induction or let it grow in the same media???




#2 bob1


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Posted 13 November 2013 - 11:56 AM

I think it is pretty stable - I was once using a Tet-inducible system (tet and dox are interchangeable) and needed to get Tet-free FBS before the system would switch off.  FBS had been through all the processing steps required to get it to the lab and it still had some activity.

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