I may have asked this before but I haven't gotten an answer and it is something that is very important for my research.
I am attempting to obtain a culture of Pseudomonas aeruginosa (PA14) electroporated with a recombinant expression vector.
I have gotten a few colonies to arise on antibiotic plates which I assumed were successful transformants however when I tried to miniprep the culture the gel shows a smear. I also tried colony pcr and that also resulted in a smear.
I want to be able to confirm that these cells indeed do have the expression vector but I am not sure how to do this if minipreps and colony PCR don't work. Does anyone have any helpful suggestions or any thoughts on why the minipreps appear as a smear (which appear even after using a brand new kit)?
Any lead would be greatly appreciated.