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stability of the FSC while sorting on a ARIA II

forward scattering fsc ARIA II

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#1 knuko



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Posted 12 November 2013 - 12:30 PM



we have up-graded our ARIA machine to an ARIA II a few month ago. Ever since then, the forward scattering is not always stable. It is either shifting slowly, or "popping" between 2 locations, that means cells laying in a nice fsc-a/ssc-a gate suddenly does not any longer. That happens even during the sortings. The drop picture does not change and looks nice. And most oddly none of the fluorescence markers/ channels is effected. Also the sort purity is fine. Just if my cells lay out side the gate, they won´t be sorted ; )

We had the BD technicians here a few times, they changed detectors for the FSC,  but the issue remains and they seem not to have a clue...

Has anyone out there seen something alike and know how to solve the issue? That would be just so great.

Thank you in advance.


regards Knut



Also tagged with one or more of these keywords: forward scattering, fsc, ARIA II

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