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stability of the FSC while sorting on a ARIA II

forward scattering fsc ARIA II

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#1 knuko



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Posted 12 November 2013 - 12:30 PM



we have up-graded our ARIA machine to an ARIA II a few month ago. Ever since then, the forward scattering is not always stable. It is either shifting slowly, or "popping" between 2 locations, that means cells laying in a nice fsc-a/ssc-a gate suddenly does not any longer. That happens even during the sortings. The drop picture does not change and looks nice. And most oddly none of the fluorescence markers/ channels is effected. Also the sort purity is fine. Just if my cells lay out side the gate, they won´t be sorted ; )

We had the BD technicians here a few times, they changed detectors for the FSC,  but the issue remains and they seem not to have a clue...

Has anyone out there seen something alike and know how to solve the issue? That would be just so great.

Thank you in advance.


regards Knut



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