i badly need your help. i had a sucessful cloning with insert in it a month ago. the clone was picked and grown in 5 ml lb with ampicillin overnight and then stored at 4 deg C. the originals plates are missing but i really need the same clone to grow again on a plate to be stored as i recently realised that is how to store the clone. so this lb with amp suspension that was sitting in 4 deg c had all the bacteria steled at bottom, i took 200 microltre and spread it on plate and left it in 37 deg c to grow overnight, am afraid all the bacteria are dead but i really need em. i was thinking, shoul i take some from this supension and try to grow in a suspension first and then plate the new suspension. or spin down this whole suspension and resuspend in new 100 microl lb and plate that or something. any suggfestions on how to get this growing will be greatly appreaciated.