I believe the protein encoded for in my cloned plasmid is phosphorylated. I've read that we can treat this protein with lambda phosphatase. the gene of this protein is cloned in a mammalian expression vector. Usually i'd transfect this plasmid into a mammalian cell line, let it incubate for 20 hours and directly harvest the cell lysate in laemlli buffer for western blot analysis.
How do i lyse the cells such that i can proceed with phosphatase treatment?