We have outsourced the production of a transfected cell line that will produce a protein of interest. My supervisor wants to know the protein production pattern of our target protein after transfection (i.e. log, linear, exponential). Since we don't have the ability to determine this ourselves, I was wondering if anyone knows a source or general rules of thumb for this type of information?
The details for a transfected cell line are as follows:
cell line = HEK293
plasmid = pcDNA 3.3
promoter = using plamsid's promoter
protein size ~55kDa
protein stability= transient
Let me know if I need to provide more information. Any help would be greatly appreciated.