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what is the difference between water-soluble and ethanol soluble hydrocortisone

culture media Greens media

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#1 roxx_gurl

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Posted 10 November 2013 - 09:19 PM

Hello everybody, 

 

I have been using hydrocortisone that been dissolved by ethanol (cheaper) despite the recipe from my supervisor is water soluble ethanol. However the result of isolated cell is not consistent, I thought my media works in beginning, but more failure than success. I am troubleshooting and wanted to check if ethanol-dissolved hydrocortisone giving me the problem. Is there anyone knows what is the difference between water-soluble and ethanol soluble hydrocortisone in term of primary cell growth?

 

Thanks 



#2 memari

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Posted 11 November 2013 - 01:27 PM

Ethanol and DMSO make micelles.


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Babak Memari

#3 roxx_gurl

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Posted 13 November 2013 - 05:39 PM

Thanks. No wonder my keratinocytes does not grow wacko.png







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