i have problems with primary prostate cells. They grew very well, but it was difficult to passage them with Accutase,and they were sticking to dish so I had to scrap them. And after passage they were growing in clusters and after 1-2 weeks begin to dye. It looked like they were not able to attach really to the dish.
Has someone some experinces with this kind of cultures? I would be gratefull for any advice.