I am having real difficulty producing a monoclonal to a particular target. When I immunise the mice I get a really got antibody titre. When it comes to fusion I can't get a positive hybridoma (seven attempts at a fusion to date). My myeloma partner is SP2 which has worked well on 8 or 9 other hybridomas I have developed over the past few months. I am absolutely mistified as to why I can't get this to work.
All ideas would be much appreciated.
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