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Hybridoma stability

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#1 Mabman



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Posted 06 May 2004 - 02:23 AM

Hi Guys
I am having real difficulty producing a monoclonal to a particular target. When I immunise the mice I get a really got antibody titre. When it comes to fusion I can't get a positive hybridoma (seven attempts at a fusion to date). My myeloma partner is SP2 which has worked well on 8 or 9 other hybridomas I have developed over the past few months. I am absolutely mistified as to why I can't get this to work.

All ideas would be much appreciated.


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