I just recently work on THP-1 as well as mammalian cell culturing.
I have a problem with THP-1 as it's non-adherent cell line, after settle it down in well, I will have to induce it with PMA. The purpose is to have differentiated THP-1 cells stick evenly after incubation. But after THP-1's settling, the cells accumulate in the center of the well, and especially after PMA treatment, cells differentiate and stick to big clumps.
Anyone have experience with this please help me!