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delivery of siRNAs with exosomes across the blood brain barrier

siRNAs exosomes rnai blood brain barrier

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#1 anaw

anaw

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Posted 06 November 2013 - 07:26 AM

Hi! my supervisor wants me to use exosomes to deliver siRNAs in our mouse model and I am trying to follow the method of Nat. Protocols El-Andaloussi et al 2012 and Alvarez-Erviti Nat Biotech 2011 but I am not getting any silencing in the brain and I have just seen these comments in you tube and in this blog talking about fraud and saying that this papers should be retracted! who should I trust? does anybody know other protocol for brain delivery of siRNAs using exosomes? I am quite desperate! any advice would be greatly appreciated!

http://www.youtube.c...h?v=Ry9kL-R-7E8

http://metamodern.co...-with-exosomes/

Cheers

Ana







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