Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

UV analysis of gel

gel gel electrophoresis

  • Please log in to reply
2 replies to this topic

#1 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 06 November 2013 - 06:11 AM

Hello,

 

I wanted to know if anyone puts their gel in a plastic bag and analyzes it under transUV after gel electrophoresis. Also, if someone could tell me what are the advantages/drawbacks of this procedure?


Cheers,

Mad Researcher

#2 jerryshelly1

jerryshelly1

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 328 posts
33
Excellent

Posted 06 November 2013 - 06:27 AM

People in our lab do if for easy cleanup. I have also seen people place a small piece of saran wrap on the illuminator before placing their gel down.

 

I think it is a waste of time, but if you are using a common lab fotodyne (for agarose, acrylamide, blots, etc.) it may be a good idea to place something down. I personally don't. I just wipe the glass down throughly.

 

Edit - It is also a pain in the ass to get a decent picture without bubbles when there is another material separating your gel from the glass.



#3 Mad researcher

Mad researcher

    Researcher in making

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 105 posts
7
Neutral

Posted 06 November 2013 - 06:31 AM

i agree to the pain in the ass part :P 

Its better to place the gel tray with the gel on the UV instead of putting it on plastic bags and then analyzing it. Just wipe up the base of the gel tray clearly before analyzing.


Cheers,

Mad Researcher





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.