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tumour dissociation - how to do it?


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#1 ClinM6

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Posted 05 November 2013 - 02:24 AM

Hi

Do anybody know working protocol for tumour (mice colon cancer) dissociation resected from mouse? I need to establish cell line.

Thanks for help in advance



#2 bob1

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Posted 05 November 2013 - 01:21 PM

Any protocol for establishment from an embryo or tissue should work well.  The usual way to go about it is to mince the tissue into small pieces, digest for some time in collagenase, then plate the bits out onto a culture surface.  You may need to play with medium formulations, FBS%, other additives depending on the tumour type.



#3 ClinM6

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Posted 05 November 2013 - 02:24 PM

Thank you so much!



#4 pcrman

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Posted 05 November 2013 - 07:42 PM

You can find detailed protocols in the attached PDF files. 

Attached File  primary_cell_culture_handbook.pdf   3.48MB   163 downloads

Attached File  Dissociation of grafted glioblastoma to isolate tumor cells.pdf   373.59KB   67 downloads






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