Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Ligation independent cloning... help!

  • Please log in to reply
No replies to this topic

#1 natashaL



  • Members
  • Pip
  • 1 posts

Posted 04 November 2013 - 01:05 PM

Just about to start using the novagen LIC kit (pET46 Ek/LIC). Does anyone know if the vector has to be prepared in advance or is it only the insert that gets treated with T4 polymerase? Also, can I transform straight into an expression vector or do I have to sub-clone first? Thanx! 

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.