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Ligation independent cloning... help!


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#1 natashaL

natashaL

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Posted 04 November 2013 - 01:05 PM

Just about to start using the novagen LIC kit (pET46 Ek/LIC). Does anyone know if the vector has to be prepared in advance or is it only the insert that gets treated with T4 polymerase? Also, can I transform straight into an expression vector or do I have to sub-clone first? Thanx! 






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