PM Sayk and see if he/she worked out the problem.
Uhm, I have asked her about it and it only worked out for her on the one day old samples, the older ones; say 2-3 days still came out bloody...The DNA pellet which should be white came out a dark brown like it was burned. I use 950 ul Cell Lysis Solution, 350 ul for all of Nuceli Lysis Solution, Isopropanol and 70% Alcohol, and 150 ul Protein Precipitation Solution. I have increased the centrifugation times and I have done the incubation step you pointed (Nuceli Lysis Solution+Protein Precipitation Solution at 4 C)
What should I do???!!!!
Edited by hercolanium, 15 November 2013 - 01:19 PM.