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Extract RNA from fixed cells

RNA extraction

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#1 cattajulio



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Posted 01 November 2013 - 04:04 PM

I fixed some primary murine  bone marrow cells with 1%PFA (10 min) and then I kept them at 4C in a solution containing 3%Newborn Calf serum, 0.02%NaAz in 1x dPBS.
I need to extract RNA from these cells for a qRT-PCR experiment.
One of my colleague has a DNA FFPE kit to extract DNA from fixed cells:I could use its Proteinase K solution.
Which buffer/ solution should I use to resuspend my fixed cells and then add Proteinase K???

I will incubate at 55C and then 80C to remove the crosslinks of the PFA. After this step  I'll use the E.Z.N.A. total RNA kit from Omega to extract RNA


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