I am working on some intronless genes. So I couldn't designed exon-intron spanning primers. I treated RNAse free DNase 37 degree for 15 min before cDNA synthesis. But my negative RT control showing amplifications i.e. positive signal after 29/30 cycles. I did qPCR twice, every time it is showing positive signal in no RT control even after DNase treatment. Does that mean my Dnase treatment is not enough and there are traces of genomic DNA contamination in my samples? But I can not design exon-intron spanning primers as I am working on intronless genes.
Can anyone help me anyway or any suggestions?