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genomic DNA extraction on silica-based membrane

DNA extraction spin column

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#1 kaveh

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Posted 31 October 2013 - 08:01 AM

I see more and more kits that use silica columns for extraction of genomic DNA from tissues, cultured cells and blood. This makes the colony screening faster and easier for mutation analysis studies or other assays. I know that one can buy the silica columns in bulk. I was wondering if anyone has recipes for required buffers (lysis buffer, binding buffer, wash buffer, elution buffer). 

 

Thank you. 



#2 bob1

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Posted 31 October 2013 - 11:32 AM

See: http://onlinelibrary...03.00397.x/full







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