Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Epigenetic drugs for cell culture


  • Please log in to reply
No replies to this topic

#1 Point Mutation

Point Mutation


  • Members
  • Pip
  • 2 posts

Posted 28 October 2013 - 12:31 PM

Can anyone recommend available inhibitors for cell culture? In particular I'm trying to find: 

  • DNA demethylation agent (I'm thinking ​5-azacytidine)
  • HDACi (maybe Vorinostat?)
  • HAT inhibitor
  • Histone demethylase inhibitor

If anyone has experience with any commercially-available products, I'd love to get some input. We want to pinpoint the mechanism of expression for our gene of interest -- it doesn't look like DNA methylation levels change (but we're going to try 5-azacytidine just to be sure) so I'm thinking it's a histone modification. I want to use inhibitors to show what will keep the gene from being expressed. Really hoping that these are all available through vendors and I'm just inept at tracking them down blink.png

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.