Why should we use 96 well plate for cytotoxicity and 6 well plate for western blot assays. For example, for MTT assay we used 96 well plate (8,000 cells/well) and obtained an EC50 as 20mcg. Now how to fix the concentrations for western blot assays? Moreover the total number of cells we used in 96 wells plate will be 8000 cells/well, but for 6 well plate we will use 1X105 cells. Is that concentration and number of cells wont correlate each other?