I have some DNA extracted by using the NaOH protocol but the yield in some is not good and all the samples are impure with 260/280 ratio ranging from 0.5-1.2
Therefore, i am planning to go for phenol chloroform extraction method but just wanted to know what are the advantages of this method over other methods (including kits)? I would be using the extracted DNA for genotyping and the source of tissue is from rat tails.
I tried running PCR's with the extracted DNA (NaOH method) but didn't get any results and i doubt it maybe because of low yield and impurities.
Looking forward for some tips and advices.
Edited by Mad researcher, 27 October 2013 - 07:19 AM.