I am currently looking for human immortalized neuronal cells. literatures I've come across thus far are on immortalized neuronal progenitor, stem cells.
May I have some recommendation?
Posted 26 October 2013 - 07:18 PM
I am currently looking for human immortalized neuronal cells. literatures I've come across thus far are on immortalized neuronal progenitor, stem cells.
May I have some recommendation?
Posted 30 October 2013 - 07:29 AM
That is not likely to be an easy find. Are you looking for a particular class of neuron (sensory, motor, cortical, etc.)?
ATCC does have a cortical neuron cell line called HCN-2 that may be worth a look. http://www.atcc.org/.../CRL-10742.aspx
What, more specifically, are you looking to do? A bit more information may help steer you in the right direction.
Posted 04 November 2013 - 04:43 AM
Posted 04 November 2013 - 10:41 AM
There are a couple of issues that are going to make it difficult to find. First, adult human tissue, especially brain tissue, is not easy to come by in general. Second, Immortalized adult neuron cell lines are essentially impossible without association with a malignancy, because neurons are by nature terminally differentiated, non-dividing cells.
I'm afraid you quite likely won't be able to find an ideal cell line, and will have to make some compromises. The closest you'll be able to get is probably to start with neural precursor cells and use a differentiation protocol to get neurons. If you have the facilities and permissions to use animals, you also might consider starting with mouse or rat neurons. There are a number of well established isolation and culture methods for a variety of model organisms.
Posted 18 November 2013 - 03:16 PM
I agree with gfischer on this; you're not going to get "immortalized neuronal cell lines", neurons are post-mitotic, thus no "cell lines" are possible. Your best bet is doing primary neuronal cell cultures (e.g, cortex), or you could consider IPS derived human neuronal cells, something like that...
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