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Using NCBI to filter results to retrieve one file for each of the several differ

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#1 luke222

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Posted 26 October 2013 - 04:06 PM

Hi I'm a third year student who is doing Bioinformatics as a module at university. I'm enjoying it and want to continue it later. Anyway my question is:

 

I was given the following questions which I'm having trouble understanding:

 

 

 

I need to use the NCBI database to retrieve ONE file for each of the several forms of ferritin found in humans. Each file should contain the  complete mRNA sequence.

 

 

 

So I went to NCBI and did a search ' Human Ferritin' and put nucleotides tab on the right.

 

I got 5038 results

 

The second result said 'Human ferritin Heavy chain mRNA complete cds'

 

I clicked on the link to display 'related sequences'

 

Brought the results to 170

 

I saw things like gorilla in the results so I want to filter even more?

 

I can click the Homosapiens filter to get it down to 72? But do I want human or homosapiens because are they not the same thing?

 

 

The results has a mixture of human and homo etc but how can I just get it to display human?

 

 

And how can I filter the results to just display 'the complete mRNA sequence'

 

 

I have done some reading and one book says' you can limit the results by excluding 'EST' as these are partial sequences but on my tab I don't have that option I just have limit STS etc

 

The question says one of the  several types of ferritin found in humans. Several means ' More than two but not many' right? but I have so many results which I can't filter down even more.

 

 

I't cant be that many because we need to compile a table and compare the mRNA sequence elements after.

 

I don't want someone to do my work for be, I want to learn how to do it myself as I'm dyslexic and have trouble interpreting questions sometimes

 

luke cool.png

 

 

 



#2 luke222

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Posted 27 October 2013 - 01:55 AM

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