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sample runs slower than ladder?

gel ladder

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#1 student47

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Posted 26 October 2013 - 12:43 PM

my sample always kind of runs a bit slower than the ladder. sample is vector around 7.3 kb which is digested and linearised, so theere might be some supercoiled and some linearised but i always have sample running around 10 kb at 30 minutes, and then at 1 hr the sample comes parallel to the 7.3 mark of ladder. why is it so?? ITS A 1% GEL IN TBE.



#2 phage434

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Posted 26 October 2013 - 01:55 PM

Variations are often caused by excess salt in the buffer used for your DNA sample. Longer run times will diffuse the salt away from the DNA sample, and may account for the more accurate response for long run times.



#3 student47

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Posted 27 October 2013 - 04:20 AM

thanks bro..







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